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Pozycja Open Access Fungal contamination in poultry waste during the industrial processing(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Oraibi, Sanaa Mahdi; Cybulska, Krystyna; Department of Chemistry, Microbiology and Biotechnology of Environment, West Pomeranian University of Technology, Szczecin; Department of Chemistry, Microbiology and Biotechnology of Environment, West Pomeranian University of Technology, SzczecinThe poultry industry produces large amounts of waste that include solid waste and waste water. Although, numerous studies have been conducted on microbial associates with various stages related to poultry and meat products processing. Only a few studies have been reported on fungal exists of poultry wastes. The aim of this study investigation of fungi associated with different poultry wastes. The sample of poultry wastes included feathers, slime, biological sludge and compost. The samples were taken at various times in peroid from 2015–2016 from different poultry slaughter houses. Isolation of fungi was performed by serial dilution and plating methods on Rose Bengal Agar (RBA), medium supplemented with antibiotic. Identification of fungi was carried out by microscopic and genetic analysis 18S ribosomal RNA gene. The number of fungi (cfu · g–1 d.m.) were isolated from chicken and goose feathers 103–104, from duck and turkey feathers even 106–107, compost 103–105, slime – 104 and biological sludge – 104–106. From the overall analysis, the dependence between the number of fungi and various type of samples and the season of taking the samples were found and type of poultry slaughter houses.Pozycja Open Access Metabolic potential of bacteria strains isolated from poultry industry waste(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Wrońska, Ilona; Cybulska, Krystyna; Department of Chemistry, Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Chemistry, Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, SzczecinMicrobial composition of poultry industry wastes was very diverse. They are inhabited by microorganisms that use organic substance to their growth. An appropriate composition of microorganisms is very important in the organic matter decomposition rate. Therefore, microorganisms that contribute to decomposition of several compounds are quite desired. The study dealt with the evaluation of metabolic activity of bacterial strains towards lipids, protein, and starch. The strains were isolated from the poultry waste environment. Metabolic potential of bacterial strains was determined based on the activity index and taking into account the hydrolysis zone diameter and colony diameter. Bacterial strains to a different degree decomposed the organic compounds. Their metabolic activity and direction towards substrates depended on the type of isolated bacterial strains.Pozycja Open Access Microbiological air rating in a variety of objects during treatment of the post-slaughter poultry wastes. Part II. Bacteria(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Oraibi, Sanaa Mahdi; Cybulska, Krystyna; Department of Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, SzczecinIn this paper, composition of the air in terms of bacterial bioaerosol in the management of post-slaughter poultry waste, was analyzed. The air samples were collected at 4 dates from 5 different locations – buildings (building for pre-processing with chemical treatment, pool for liquid waste, facility for storage of sludge from biological sewage treatment, object for preparation of waste subject to composting, and proper composting facility). Analyses were carried out in accordance with the procedures of environmental microbiology. Number of total bacteria was determined with particular emphasis put to Pseudomonas sp. and Clostridium sp. as well as Actinomycetes sp. Based on the air assays, it was found that the bacterial bioaerosol was present in varying degrees depending on the selected groups of microorganisms and differed between dates of analyzes and research points i.e. type and purpose of the object for these wastes management.