Wydawnictwo Uczelniane

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    Analysis of the Myostatin Gene (MSTN) Polymorphism in Four Breeds of Horses
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Polasik, Daniel; Pikuła, Ryszard; Gawlik, Justyna; Ochman, Joanna; Terman, Arkadiusz; Department of Genetics and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland; Laboratory of Horse Breeding and Animal-Assisted Therapy, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland
    Gene encoding myostatin – MSTN is a marker for race distance, speed indices and body composition in Thoroughbred racehorses . Because it shows high variability depends on breed and utility type the aim of this study was to: design PCR based test for g.66493737C/T polymorphism analysis in intron 1 of the equine MSTN gene; analyze occurrence of g.66493737C/T variants in four breeds of horses, raised in Poland. Investigations were carried out on 209 horses belong to the following breeds: Holstein, Polish Noble Half-breed, Polish Heavy Draft and Polish Konik. For MSTN gene analysis amplification created restriction site method (ACRS) was applied. Digestion of MSTN gene amplicons (132bp) by RsaI enzyme allowed to discriminate individual genotypes based on following restriction fragments lengths: TT – 132bp, CT – 132, 103, 29bp and CC – 103, 29bp. Obtained results showed that CC genotype (speedy, sprint type) was present only in Holstein breed (0.073), which is known to have English Thoroughbreds ancestors. CT genotype (fast, middle-distance type) appeared with highest frequency (0.735) in Polish Noble Half-breed horses. Both of breeds represents saddle type. Last genotype – TT (greater stamina) was most common in Polish Heavy Draft (0.830) and Polish Konik (0.736) horses which are classified to heavy-draught and general type respectively. Differences in genotypes frequencies between breeds were statistically significant (p ≤ 0.05 for CC, p ≤ 0.01 for TT and p ≤ 0.001 for CT). Results of this study showed MSTN gene variants differentiation depends on utility type and breed of horses raised in Poland.