Wydanie 316(33)1 2015

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https://oa.zut.edu.pl/handle/20.500.12539/716

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    PozycjaOpen Access
    The First Molecular Characterisation of Steinernema Silvaticum Recorded in Poland and its Differentiation from Steinernema Kraussei Using Ribosomal DNA (rDNA) Sequences
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Dzięgielewska, Magdalena; Berdzik, Marcin; Myśków, Beata; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland
    During faunistic studies conducted in north-western Poland on the entomopathogenic nematodes (EPN) of the families Steinernematidae and Heterorhabditidae, species Steinernema silvaticum was identified for the first time in Poland. Nematode species identification, using morphometric methods, is laborious, even for an experienced researcher. S. silvaticum and S. kraussei are defined as “sister taxa” and it is difficult to distinguish these two species. The study is an attempt to differentiate S. silvaticum from S. kraussei using sequencing results of the rDNA – ITS1 and LSU regions. Sequenced regions of ITS1 and LSU had lengths of 490bp and 918bp, respectively. A very high similarity between the S. silvaticum isolate and the isolate of S. kraussei was detected in the case of both sequences (98–99%). There were only two nucleotide differences in ITS1 and two in LSU region, which discriminate the two analysed species.