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Pozycja Open Access Alleviating Effects of Ascorbic Acid on Lead Toxicity in Goji (Lycium barbarum L.) in Vitro(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Krupa-Małkiewicz, Marcelina; Kruczek, Arleta; Pelc, Justyna; Smolik, Beata; Ochmian, Ireneusz; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, PolandLead (Pb) is the most common heavy metal pollutant in the environment. The objective of the presented study was to investigate the ameliorative effect of exogenous 1 mM ASA on key growth and biochemical parameters in Lycium barbarum seedlings under heavy metal (Pb(NO3)2) stress in vitro. Nodal cutting with an axillaries bud were used as an explants. The results showed that lead accumulation in goji explants had negative influence on morphological parameters of plant growth, such as shoot and root length. Lead caused a significant reduction in chlorophylls and carotenoid content, increased lipid peroxidation and induced significant accumulation of proline in goji leaves. Addition to MS medium 1 mM ASA greatly alleviated Pb-induced growth inhibition and Pb-induced MDA and proline accumulation. Presence of ASA in the MS medium under heavy metal stress increased plant fresh and dry mass with no significant effect on plant water content.