Genotypic Differences between Tomato Cultivars Differing in Their Response to Salinity Stress
Ładowanie...
Pliki
Data
2014
Tytuł czasopisma
ISSN czasopisma
Tytuł tomu
Wydawca
Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie
Abstrakt
Celem pracy było określenie różnic genotypowych między trzema odmianami
uprawnymi pomidora [Lycopersicon esculentum (Mill.)] – Zorza, Paw i Złoty Ożarowski, poddanych
działaniu stresu solnego w warunkach laboratoryjnych. Tolerancję na zasolenie określono za
pomocą testu szalkowego na płytkach Petriego, stosując trzy stężenia soli: 25 mM · dm–3 NaCl +
25 mM · dm–3 KCl, 25 mM · dm–3 NaCl + 50mM · dm–3 KCl oraz 50 mM · dm–3 NaCl. Kontrolę
w doświadczeniu stanowiła woda sterylna. Na podstawie przeprowadzonych pomiarów
biometrycznych (zdolność kiełkowania, długość siewki i korzeni), badane genotypy podzielono
na trzy klasy fenotypowe według ich cech morfologicznych. Siewki uznane za tolerancyjne
przydzielono do pierwszej klasy fenotypowej, średniotolerancyjne do drugiej, a wrażliwe do
klasy trzeciej. Różnice genotypowe pomiędzy badanymi obiektami określono, stosując technikę
ISSR-PCR. Zaobserwowano różnice w długości amplifikowanych sekwencji miedzymikrosatelitarnych
pomiędzy badanymi odmiana pomidora, jak i siewkami należącymi do różnych klas fenotypowych.
Podobieństwo genetyczne między odmianą Paw i Złoty Ożarowski wynosiło 45,2%, a między
odmianą Zorza i Paw – 54,8%.
The aim of the study was to determine genotypic differences between three tomato [Lycopersicon esculentum (Mill.)] cultivars – Zorza, Paw and Złoty Ożarowski which were subjected to salinity stress under laboratory conditions. The plant response to salinity was determined by means of a pan test on Petri dishes, using three concentrations of salt: 25 mM · dm–3 NaCl + 25 mM · dm–3 KCl, 25 mM · dm–3 NaCl + 50 mM · dm–3 KCl and 50 mM · dm–3 NaCl. Sterile water was used as the control. On the basis of the biometric measurements (the number of germinating seeds, seedling’s height, and the length of seedling’s roots), each genotypes were divided into three phenotypic classes. The tolerant seedlings were classified to the first phenotypic class, the medium-tolerant to the second one, and the susceptible to the third class.The genotypic differences between cultivars investigated were described using the ISSR-PCR technique. It has been found that not only tomato cultivars, but also seedlings from different classes differed in respect to length of sequences amplified located in the inter simple sequence repeats regions of tomato genomes, and genetic similarity described for cultivars Paw and Złoty Ożarowski was 45.2%, and for cultivars Zorza and Paw – 54.8%.
The aim of the study was to determine genotypic differences between three tomato [Lycopersicon esculentum (Mill.)] cultivars – Zorza, Paw and Złoty Ożarowski which were subjected to salinity stress under laboratory conditions. The plant response to salinity was determined by means of a pan test on Petri dishes, using three concentrations of salt: 25 mM · dm–3 NaCl + 25 mM · dm–3 KCl, 25 mM · dm–3 NaCl + 50 mM · dm–3 KCl and 50 mM · dm–3 NaCl. Sterile water was used as the control. On the basis of the biometric measurements (the number of germinating seeds, seedling’s height, and the length of seedling’s roots), each genotypes were divided into three phenotypic classes. The tolerant seedlings were classified to the first phenotypic class, the medium-tolerant to the second one, and the susceptible to the third class.The genotypic differences between cultivars investigated were described using the ISSR-PCR technique. It has been found that not only tomato cultivars, but also seedlings from different classes differed in respect to length of sequences amplified located in the inter simple sequence repeats regions of tomato genomes, and genetic similarity described for cultivars Paw and Złoty Ożarowski was 45.2%, and for cultivars Zorza and Paw – 54.8%.
Opis
Słowa kluczowe
genotypes, ISSR-PCR, Lycopersicon esculentum, response, salinity, genotypy, reakcja, zasolenie, SSR-PCR, Lycopersicon esculentum
Cytowanie
Krupa-Małkiewicz M., Franczak M., Grabiec M., Smolik B., Smolik M. (2014). Genotypic Differences between Tomato Cultivars Differing in Their Response to Salinity Stress. Folia Pomer. Univ. Technol. Stetin. Agric., Aliment., Pisc., Zootech. 309 (29), 75–84