Wydanie 322(36)4 2015

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https://oa.zut.edu.pl/handle/20.500.12539/712

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    PozycjaOpen Access
    Microstructure of the Masseter Muscle (Musculus Masseter) in Cattle
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Bogucka, Joanna; Bogucki, Mariusz; Frymarski, Michał; Department of Animal Biochemistry and Biotechnology, UTP University of Science and Technology in Bydgoszcz, Poland; Department of Cattle Breeding, UTP University of Science and Technology in Bydgoszcz, Poland; Department of Animal Biochemistry and Biotechnology, UTP University of Science and Technology in Bydgoszcz, Poland
    The aim of the study was to examine the microstructure of the masseter muscle (musculus masseter) in slaughter cattle depending on sex and age. The experiment used 40 cattle (7 cows more than 5 years old, 12 heifers and 21 bulls less than 24 months old). Following slaughter, muscle samples were collected and frozen in liquid nitrogen. Frozen samples were to cut into 10 μm sections on a cryostat. Next, the sections were placed on a glass slide and stained for NADH-TR activity to identify oxidative (βR) and glycolytic (αW) muscle fibres. Age and sex of cattle had a significant effect on the diameter of muscle fibres. Older animals had a greater diameter of both fibre types. In females βR and αW fibres had a greater diameter than in males. The masseter muscle contained a greater percentage of oxidative compared to glycolytic fibres. The age of the studied animals had no effect on the content of different muscle fibre types and the fibre proportions in young and adult slaughter cattle were at a similar level. Sex of animals was found to have an effect on the percentage of βR and αW fibres. A significantly greater proportion of oxidative fibres in the masseter muscle was found in males. An inverse relation occurred for glycolytic fibres, which were more abundant in the muscles of females.