Wydanie 341(46)2 2018

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https://oa.zut.edu.pl/handle/20.500.12539/698

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    PozycjaOpen Access
    In Vitro Selection of Solanum Pimpinellifolium Plant Tolerant to NaCl
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Krupa-Małkiewicz, Marcelina; Kulpa, Danuta; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland
    Salinity is a major abiotic stress for plant worldwide which can reduce the average yields of most major crops such as tomato by more than 50%. The response of tomato to salinity is variable depending upon the line or cultivar. The aim of this study was carried out to determine the variation in salt tolerance for wild genotypes of tomato S. pimpinellifolium. To initiate callus tissue different combination of plant growth regulators were added to MS medium. The tolerant forms were selected at the callus stage and the stage of plants regenerated through somatic embryogenesis. Callus culture and shoot explants were exposed to different levels of salinity stress ranging from 0 (control) to 25, 50, 75, 100, 125 and 150 mM NaCl. The highest weight of dark green colour callus was observed on MS medium supplemented with 2.0 mg · dm–3 BAP and 2.0 mg · dm–3 IAA. It was shown that salt stress affected all growth parameters and addition to the MS medium 125 and 150 mM NaCl inhibited callus and somatic embryo initiation. The results obtained in this study suggested that S. pimpinellifolium somaclones isolated from callus selected on MS medium supplemented with 100 mM NaCl showed highest tolerance to salt stress.