Przeglądaj wg Autor "Shuvar, Ivan"

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  • Miniatura
    PozycjaOpen Access
    Dilated Cardiomyopathy in the Domestic Dog (Canis Lupus Familiaris) – in Silico Analysis of Selected Genes
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2022) Wilim, Daria; Gruszczyńska, Joanna; Grzegrzółka, Beata; Shuvar, Ivan; Scientific Association of Experimental and Laboratory Animals, Warsaw University of Life Sciences – SGGW, Warszawa, Poland; Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences – SGGW, Warszawa, Poland; Scientific Association of Experimental and Laboratory Animals, Warsaw University of Life Sciences – SGGW, Warszawa, Poland; Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences – SGGW, Warszawa, Poland; Lviv National Agrarian University, Dublyany, Lviv, Ukraine
    Dilated cardiomyopathy (DCM) is a progressive loss of contractility of the heart muscle as the disease progresses. It causes a decrease in the heart’s minute capacity, i.e. the volume of blood pumped by the heart into the blood vessels in one minute. DCM leads to congestive heart failure and sudden death. The aim of this study was to identify in silico genes within which mutations have occurred that may cause DCM in the domestic dog (Canis lupus familiaris), to identify dog breeds at risk, and to propose breed-specific diagnostic molecular tests. For bioinformatic analyses of sequences re trieved from GenBank (NC_006587.3 – FGGY, NC_006583.3 – DCC and CM023383.1 – PDE3B) and from scientific publications (PDK4 – from patent publication number US 2011/0307965 A1 and STRN – Meurs et al. 2010), the following programs were used: Primer3 v. 0.4.0, NEBcutter v. 2.0 and BLAST. Based on literature data, domestic dog breeds such as Doberman Pinscher, Boxer, Portuguese Water Dog, Newfoundland, Irish Wolfhound and Great Dane were found to be among the breeds with the highest risk of DCM. In order to identify relevant mutations in the genes studied (FGGY, DCC, PDE3B, PDK4 and STRN) that may cause the occurrence of dilated cardiomyopathy, the use of specific restriction enzymes has been proposed in molecular diagnostic tests: BmiI for mutations in the PDK4 gene and Tth111I for SNPs in the FGGY gene (Doberman Pinscher) and TaqI for SNPs in the DCC gene and HinfI for SNPs in the PDE3B gene (Irish Wolfhound). This work, may serve as a prelude to analysis for targeted genetic testing to enable correct diagnosis of DCM in asymptomatic dogs.
  • Miniatura
    PozycjaOpen Access
    Herbological Condition and Herbicide Control of Potato Agrophytocenosis in the Western Part of Ukraine
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2020) Shuvar, Ivan; Korpita, Hanna; Lviv National Agrarian University, Dubliany, Ukraine
    The article presents original research on the herbicides influence on weed species and productivity of potato agrophytocenosis of Volya variety under organo-mineral and organic fertilizer systems in the western part of Ukraine.
  • Miniatura
    PozycjaOpen Access
    Potential Contamination of Soybean Agrophytocenosis Depending on the System of Farming and Soil Tillage
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2020) Tsyuk, Oleksiy; Marchenko, Dmutro; Shuvar, Ivan; Biel, Wioletta; Department of Agriculture and Herbology, National University of Life and Environmental Scienсes of Ukraine; Department of Agriculture and Herbology, National University of Life and Environmental Scienсes of Ukraine; Agriculture and Livestock Department, Lviv National Agrarian University, Dubliany, Ukraine; Department of Monogastric Animal Sciences, Division of Animal and Food, West Pomeranian University of Technology in Szczecin, Poland
    This work presents the results of the study of changes of the potential contamination of soybean agrophytocenosis in grain-row crop rotation by using different systems of farming and typical black soil tillage. It was established that in the upper 0–5 cm layer of soil there are on average 393–671 million pieces of weed seeds. which is 33.6–43.0% of the total number in the layer of soil 0–20 cm. It was established that the use of periodical moldboard and superficial basic soil tillage had no impact on reducing the potential contamination of typical black soil compared to the variant of differentiated tillage. During subsurface plowing the total number of seeds in the soil layer 0–20 cm increased by 13.5–25.8% compared to differentiated tillage. In the variants of ecological and biological farming systems the number of weed seeds in the layer 0–5 cm was 11.3–19.6% higher compared to the intensive farming system. Based on monitoring of the species composition of the weed seeds bank it was established that in the arable layer of the soil the largest share are annual dicotyledonous – 48–62%. annual monocotyledonous – 30.2–44.6%. perennial – 0.9–3.3% of the total number. It was proved that under the intensive system of farming the yield of soybean variety Silesia increased on average from 10.2 to 147% compared to the ecological and biological system of farming. During the biological system of farming soybean yield did not exceed 1.0–1.8 t/ha. Therefore. the industrial and ecological system of farming had the greatest impact on reducing the potential weediness of the soybean agrocenosis during the growing season. During the biological system of farming crop yields decrease due to the accumulation of weed seeds in the topsoil and formation of high actual weediness of the soybean agrocenosis.