Przeglądaj wg Autor "Dobrowolski, Jan"

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    Kompleksometryczne oznaczanie wapnia i magnezu w surowicy krwi przy użyciu wskaźników mieszanych
    (1959) Dobrowolski, Jan; Świerczewska, Maria; Wyższa Szkoła Rolnicza w Szczecinie. Zakład Biochemii; Pomorska Akademia Medyczna w Szczecinie. Katedra Chemii Ogólnej
    Complexometric determination of calcium and magnesium in blood serum was introduced by applying the glycocol buffer and the compound indicators prepared according to our own prescription. Addition of dye, acting as the background for the indicator proper, results in a more distinct change of colour of the solution in the end point of titration. For determination a solution of 0,001 N and 0,01 N disodium versenate was applied to a mixture of indicators consisting of: eriochrome black T. with napthol yellow (ratio 1 :1) for calcium and magnesium, and mixture of murexide with napthol green В (1 :1) for calcium. Titrations was performed in a medium of pH=10,0 (with eriochrome black T.) and pH = 12,0 (with murexide) and the buffer consisting of 0,1 n glycocol solution and 0,1 n NaOH was applied. The numerical analysis of results obtained during the investigations allows to draw the conclusion that the direct titration may be performed only with no less anamount than 0,5 ml of the blood serum. The less volumes give always the excess error. Blood — plasma proteins may be the cause of the titration error. More accurate results are obtained by precipitation of calcium as calcium oxalate. Analysing volumes of blood serum greater than 0,5 ml and properly diluted the accuracy of approximately ±1 percent for calcium and ±2 percent for magnesium may be obtained.