Wpływ roślinnych regulatorów wzrostu (2,4-D, BAP, GA3) na namnażanie pędów i tworzenie tkanki kalusowej bazylii pospolitej (Ocimum basilicum L.)

Abstract

The aim of this study was an attempt to determine the influence of PGRs – auxins, cytokinins and gibberelic acid – on the propagation and callus tissue of sweet basil (Ocimum basilicum L.). The best result at the propagation stage were obtained on MS medium supplemented with 1.0 mg · dm–3 BAP with 0.1 mg · dm–3 GA3. The basilicum plants propagated under these conditions developed high main shoots and large number of shoots in comparison to control. The leaves with the best callus weight were regenerated on MS medium supplemented with 1.0 mg · dm–3 2,4-D and 0.5 mg · dm–3 BAP.