The results of allergic, bacteriological and biochemical studies in the diagnosis of
mycobacterial infections of mammals and birds in the zoo of the municipal enterprise
«T. H. Shevchenko park of Culture and Rest» Dnipro city are described. Comprehensive
epizootic research was aimed at the exclusion of dangerous pathogenic mycobacteria. Zoological
zones of pathogenic mycobacteria were not recorded in the environment and in animal
organisms, but in the ground of enclosures and faeces we identified three cultures of acid-fast
microorganisms, one of them was identified as M. flavescens, indicating the circulations of
mycobacterial agents in internal and external environment of animal biotope of zoo.