Zastosowanie metod RAPD i ISSR do oceny mieszańców pszenżyta z Aegilops crassa 4x Boiss

Abstract

In the study the genetic similarity in triticale (6x) hybrids with Aegilops crassa (4x) Boiss was estimated and existence of DNA fragments of the wild species in the triticale (6x) hybrid strains background was confirmed. Seventeen RAPD and fourteen ISSR selected primers were used in analyses. A total number of 152 bands RAPD and 220 ISSR products were obtained, out of which 34% were polymorphic in the both cases. In hybrids fourteen RAPD and twenty ISSR fragments specific for Aegilops crassa (4x) Boiss were identified (9% in the both cases). Both methods allowed to confirm the hybrid character of all the strains estimated. Based on the polymorphism of RAPD and ISSR markers, the values of Dice genetic similarity indices between the pairs of genotypes studied were calculated. Mean genetic similarity between the hybrid forms and triticale parental components was the same for both methods used – 0,88. Most different from the others was the maternal species Aegilops crassa 4x Boiss. Dendrograms showing relatedness between analyzed populations were constructed based on Dice’s similarities.