Wydanie 340(45)1 2018
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Przeglądaj Wydanie 340(45)1 2018 wg Temat "bioaerozol"
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Pozycja Open Access Contamination of Bioaerosol in the Confectionery Facility with Enterobacteriaceae Members(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kręcidło, Magdalena; Krzyśko-Łupicka, Teresa; Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland; Independent Department of Biotechnology and Molecular Biology, University of Opole, PolandGood manufacturing practice includes a monitoring of purity in the production environment. It is important to estimate the source of microbial contamination and the influence of microclimatic conditions on changes in the microbial population of the air. The aim of study was to assess the occurrence of Enterobacteriaceae in composition of bacteriological bioaerosol from technological halls during production. The study were conducted in the confectionary facility in two technological halls (H.1, H.2) during production. In each hall, where the different stages of production were ran and various number of workers were worked, was determined 7 sampling sites. The air samples were taken by volumetric method with using MAS-100 impactor. Petri dishes were incubated in the standard conditions for the aerobic bacteria (22°C, 72 h). Isolates were stained by Gram’s technique and the members of Enterobacteriaceae were diversified by analysis the biochemical properties. Two-way ANOVA tests were performed to analyse numer of bacteria as a function of sampling site and production hall. The results were given as CFU ∙ 1 m–3 of air. Statistical analysis were performed in the R Studio. The number of workers had positive correlations with the concentrations of bacterial bioaerosols. Enterobacteriaceae dominated in the sectors with the higher number of workers i.e. in sector hand – sorting and packaging of finished products.