Przeglądaj wg Autor "Gaczarzewicz, Dariusz"

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    Oxygraphic evaluation of activity of respiratory enzymes in boar's semen
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Kopczyński, Piotr; Lasota, Bogdan; Ogoński, Tadeusz; Stankiewicz, Tomasz; Gaczarzewicz, Dariusz; Skolmowska, Magdalena; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin
    In the article advantages of the use of oxygraphic method for metabolic examination of mitochondrial respiratory enzymes in boar spermatozoa were described. Investigations were performed on semen samples taken from 36 boars of 4 breeds, at the age from 8 to 40 months. All samples of semen characterized at least 80% content of motile spermatozoa. The rate of oxygen uptake was measured with the use of Clark type electrode connected with analog-digital recorder. Measurements were executed in temperature 37°C, and the rate of oxygen uptake (v) was expressed in nmol O2 · min–1 per 108 spermatozoa. The values of oxygen consumption rate after application of succinate (vs) and PMS (vp) made up the basis of analysis. The results show that the method of oxygraphic evaluation of spermatozoa movement system described in the paper makes possible the precise investigation of the spermatozoa respiratory chain status and enables selection of boars with the best parameters of semen. This method can be taken in consideration at semen examination in the course of boar's selection for the AI stations, after boar's reproductive disease, in periodical semen tests and also when taking decision about the boar's elimination.