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    Effect of Carbohydrate Source on In Vitro Propagation and Rooting of Two Cultivars of Petunia (Petunia × Atkinsiana D. Don)
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Krupa-Małkiewicz, Marcelina; Fornal, Natalia; Kosatka, Alicja; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland
    The effects of carbohydrate type and concentration on shoot multiplication of two cultivars of Petunia × atkinsiana D. Don ‘Prism White’ and ‘Prism Rose’ and on the root formation in in vitro culture were investigated. Sucrose, fructose, glucose, and maltose were tested at concentration ranging from 0 to 50 g · dm–3. Glucose and maltose did not stimulate proliferation of petunia shoots. Either the lack of carbon source or its high concentration (50 g · dm–3) was completely ineffective in rooting induction for both cvs. ‘Prism White’ and ‘Prism Rose’. In ‘Prism White’ the highest mean values for microshoot number together with the highest number of new shoots per one explant was obtained on the medium containing 30 g · dm–3 of fructose. Considering ‘Prism Rose’, the more favorable sugar source and concentration for shoot length was obtained on medium with 30 g · dm–3 of sucrose. The highest number of adventitious roots produced per shoot of petunia ‘Prism White’ was obtained on medium supplemented with 40 g · dm–3 of glucose and for petunia ‘Prism Rose’ – 30 g · dm–3 sucrose and maltose. The results presented in this study indicate that sucrose could be replaced by other carbon source in micropropagation of petunia.