Wydanie 326(38)2 2016

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https://oa.zut.edu.pl/handle/20.500.12539/709

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Przeglądaj Wydanie 326(38)2 2016 wg Autor "Department of Animal Anatomy, Institute of Veterinary Science University of Agriculture in Krakow"

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    PozycjaOpen Access
    Preliminary research on evaluation of sperm morphometry and chromatin structure in the semen of silver fox (Vulpes vulpes)
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Andraszek, Katarzyna; Szeleszczuk, Olga; Niedbała, Piotr; Kuchta-Gładysz, Marta; Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding Siedlce University of Natural Sciences and Humanities; Department of Animal Anatomy, Institute of Veterinary Science University of Agriculture in Krakow; Department of Swine and Small Ruminant Breeding, Institute of Animal Science University of Agriculture in Krakow; Department of Animal Anatomy, Institute of Veterinary Science University of Agriculture in Krakow
    Semen analysis is a way to predict male fertility. The quality of sperm morphology evaluation depends on the diligence put in the preparation of smears, their fixation and cell staining, since these may affect the morphometry of the head and the entire sperm alike. The aim of the study was a detailed morphometric analysis and chromatin structure evaluation in the sperm cells of the silver fox (Vulpes vulpes). The material involved the sperm of year-old captive silver foxes. The slides were stained using one of the following dyes: aniline blue (AB), chromomycin (CMA3), and acridine orange (AO). The staining revealed prevailing sperm with normal histone retention. The measurements resulted in the following mean values of the fox sperm morphometric parameters: head length and width: 6.59 μm and 4.39 μm, respectively, head perimeter: 17.98 μm, head area: 21.69 μm2, acrosome area: 11.2 μm2, acrosome coverage: 51.69%, mid-piece length: 12.84 μm, mid-piece coverage: 19.72%, tail length: 65.11 μm, sperm length: 71.70 μm, head ellipticity index: 1.51, head elongation: 0.2, head roughness: 0.84, and head regularity: 1.05. According to the literature on differential sperm staining for morphological evaluation purposes, the lack of established standards and the use of a variety of dyeing techniques appear as a current issue.