Wydanie 340(45)1 2018

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https://oa.zut.edu.pl/handle/20.500.12539/699

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Przeglądaj Wydanie 340(45)1 2018 wg Autor "Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland"

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  • Miniatura
    PozycjaOpen Access
    Contamination of Bioaerosol in the Confectionery Facility with Enterobacteriaceae Members
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kręcidło, Magdalena; Krzyśko-Łupicka, Teresa; Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland; Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland
    Good manufacturing practice includes a monitoring of purity in the production environment. It is important to estimate the source of microbial contamination and the influence of microclimatic conditions on changes in the microbial population of the air. The aim of study was to assess the occurrence of Enterobacteriaceae in composition of bacteriological bioaerosol from technological halls during production. The study were conducted in the confectionary facility in two technological halls (H.1, H.2) during production. In each hall, where the different stages of production were ran and various number of workers were worked, was determined 7 sampling sites. The air samples were taken by volumetric method with using MAS-100 impactor. Petri dishes were incubated in the standard conditions for the aerobic bacteria (22°C, 72 h). Isolates were stained by Gram’s technique and the members of Enterobacteriaceae were diversified by analysis the biochemical properties. Two-way ANOVA tests were performed to analyse numer of bacteria as a function of sampling site and production hall. The results were given as CFU ∙ 1 m–3 of air. Statistical analysis were performed in the R Studio. The number of workers had positive correlations with the concentrations of bacterial bioaerosols. Enterobacteriaceae dominated in the sectors with the higher number of workers i.e. in sector hand – sorting and packaging of finished products.
  • Miniatura
    PozycjaOpen Access
    Seasonal Changes in Occurrence of Fusarium Isolates in Grain Warehouses
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kręcidło, Łukasz; Krzyśko-Łupicka, Teresa; Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland; Independent Department of Biotechnology and Molecular Biology, University of Opole, Poland
    Cereal grains on global scale provide more energy than other types of crops. They are a rich in carbohydrates and are the main source of this nutritional substances. Cereal and products made of it supply half of daily carbohydrate intake for average person. Over 80% of the worldwide grain production constitutes maize, wheat and rice. The main crops cultivated in Poland are wheat, rye, barley, oat and maize, because there are most conformed to the climate. The chain of agri-food production might be divided into three stages: the cultivation period, storage time and processing activities. At each of these stages there are different hazards in the production process including mycological contaminations. An severe issue is the contamination by the Fusarium species and production of mycotoxins by them. Fusarium species are capable of infecting the cultivated crops and may lead to decline the grain quality. The aim of this study was to determine the degree of contamination of grains and the storage area by Fusarium species during four storage periods. The study was conducted in four periods of research in two warehouses. The analysis included following assessments: the composition of the mycological bioaerosol from the warehouses, the number of microorganisms on the walls of the warehouses, the number of fungi in the fraction which settle on the surfaces of the warehouses and the microbiological quality of stored grain. Fusarium species were an insignificant part of the isolates from the surfaces of the warehouses. Microbiological quality of stored grain depended on the raw material and was different in each of the warehouses. The degree of grain contamination by Fusarium species decreased during the storage period.