The Role of Staining Techniques in Seminological Analysis of Mammalian Semen

Ładowanie...
Miniatura

Data

2015

Tytuł czasopisma

ISSN czasopisma

Tytuł tomu

Wydawca

Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie

Abstrakt

The most important semen parameters are the concentration, motility and morphology of sperm cells. Sperm morphology is regarded as the most reliable parameter for predicting fertility in males. A problem in evaluating sperm morphology and morphometry is the lack of standardization of staining techniques. The staining procedure and reagents used can significantly affect the morphometric parameters of the sperm cell. The use of stains with different pH or osmotic pressure, as well as the duration of the procedure, may influence the shape and size of the sperm, and thus the result of the morphological evaluation of the semen. It is necessary to develop an evaluation procedure for sperm morphology and morphometry that will minimize the changes in the structure of the evaluated semen in relation to the native semen.

Opis

Słowa kluczowe

morphology, morphometry, semen, spermatozoa, staining techniques, morfologia, morfometria, nasienie, plemnik, techniki barwienia

Cytowanie

Banaszewka D., Andraszek K., Zdrowowicz E., Czubaszek M., Walczak-Jędrzejowska R. (2015). The Role of Staining Techniques in Seminological Analysis of Mammalian Semen. Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Zootech. 320(35)3, 5–20